Bd facsdiva software basic experiment guide please note, this guide is not intended to take the place of inperson training but is to be used as a reference as needed after completing training with unified flow core staff. Stanford lsrfortessa user guide 100710 starting up 1. Additional laser choices are available and may be configured at the time of purchase or at a later date to meet evolving application. The software calculates the voltage necessary to set the baseline at 10x sden. Bd lsr ii flow cytometer bd customer education provides comprehensive training on bd instruments and software. I dont have an lsr, but an ariaii so some of the specifics will be different, but the principles the same. The online help installed with your bd facsdiva software contains the. The bd lsrfortessa x20 cell analyzer delivers highperformance multicolor analysis with the most compact footprint in its class at just 30 x 29 76. The lsr fortessa offers 5 lasers and is able to detect up to 18 parameters fluorochromes at the same time. Bd lsrfortessa cell analyzer technical specifications the bd lsrfortessa cell analyzer puts the power of the. Flow cytometry service bd lsr fortessa sorp how to use guide 5 4 create a new specimen click the icon with a syringe. Compensation of a 7 color panel on the bd lsr ii youtube.
We use saline as a sheath fluid, which is in 20 litre facsflow boxes under the benches behind the machine. The rockefeller university bd lsriis and bd lsrfortessa. Bd lsrfortessas two optically identical instruments are equipped with four lasers 405nm, 488nm, 561nm, 640nm. It can be used for routine research applications, immunophenotyping, dna cell cycle, calcium flux analysis. The bd lsrfortessa cell analyzer offers the ultimate in choice for flow. General rules on instrument care faculty of medicine flow. Please check with your local sales representative for the latest wavelength and power options. Log in to diva at the prompt with your diva username and password. Bd lsrfortessa users guide facsdiva software reference manual. Bd ran with this idea, and implemented a different method in their cytometry setup and tracking. Any use of this product other than the permitted use without the express written authorization of becton, dickinson and company is strictly prohibited.
Bd lsrfortessa 18color 20181211 rockefeller university. Bd lsr fortessa flow cytometry core facility university of bergen. The bd lsr fortessa x20 cell analyzer delivers highperformance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers. Our instrument laboratory provides training and consultation services for researchers whose work involves flow cytometry, confocal microscopy and geldocumentation. The bd lsrfortessa analyzer can be configured with up to 4 lasersblue, red, violet, and uvwhich enables the detection of up to 18 colors simultaneously. The panel was used for defining tcell subsets using lysed whole blood bd pharm. In our studies, we were able to look at t cells, b cells, monocytes, macrophages, neutrophils, and dendritic. If facsdiva software alerts you to either problem, you can leave the machine on standby to replace sheathempty waste. Bd lsrfortessa configuration immunophenotyping core. Lsrfortessa x20 analyzer md anderson cancer center.
The bd lsriis and bd lsrfortessa can be configured with up to five fixed alignment lasers. Reading this material and answering the questions at the end of each. Power panel figure a5 facsdiva icon figure a4 control panel figure a2 sheath filter figure a3 roller clamp. Bd lsriis and bd lsrfortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer. It can be used to detect up to 17 fluorescent parameters. The bd lsr fortessa x20 cell analyzer is a great instrument for looking at multiple cell markers within a sample. Brc sorp fortessa 5 laser, 20 parameter sorp instruments 355 nm nuv, 405 nm violet, 488nm blue, 561nm yellowgreen, 633 nm red, hts option for 96 well plate acquisition. The bd lsrfortessa may be upgraded subsequently with additional or new lasers from bd, as future requirements dictate. The software is common across bd facs instrument platforms, including the bd facscanto cell analyzer and bd facsaria cell sorter systems. Designed for limited space and boundless potential. The bd lsrfortessa x20 can be configured with up to 5 lasers, which enables the detection of up to 20 parameters simultaneously. The fortessas run at the same speed with the same software as the cantoii, but has the fluidics system of the facscalbur.
The bd facscelesta multicolor cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package. This video describes the process of manual and auto compensation in. In addition to the bd high throughput sampler, single tube acquisition is also possible. Its primary function is to characterize complex populations of cells. The bd lsriis and bd lsrfortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. It uses fixedalignment lasers that transmit light through a flow cell to configurable octagon and trigon detector arrays.
First, bd has a diva software tutorial you should have gotten a copy with the instrument. Bd lsr fortessa x20 cell analyzer biocompare product. The bd lsr ii is the first and only aircooled, four laser benchtop flow cytometer with the ability to acquire up to 18 colors. Zip, weve seen laser linespecific signal issues on our fortessa, and in our case it usually shows up when you try to acquire immediately after priming or leave the instrument running without a tube for an extended time the green and blue are on opposite ends of the pinholes, if we run a time vs. The fortessa has a 405nm, 488nm, 561nm and 633nm laser setup with the ability to detect signals in 16 channels. The bd lsrfortessa x20 cell analyzer delivers high performance multicolor analysis with the most compact footprint in its class. This instrument is for analysis only and has five lasers uv, violet, blue, green and red with 16 fluorescence detectors two from uv laser, four from violet laser, two from blue laser, five from green laser, three from red laser and is custom configurable by. At the heart of the bd lsr ii is the new digital acquisition, allowing for additional parameters to be detected, and yielding more information per cell.
Run bd facsdiva software by doubleclicking the software icon on the desktop fig. Bd facsdiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the bd lsrfortessa workstation. The most common laser choices are blue, red, violet, yellowgreen, and uv. The rockefeller university bd lsrii1 uv laser operation. Bd lsrfortessa special order research program offers the latest innovations in laser technology to researchers to enable future upgrades with new excitation. Cytek aurora next level spectral flow cytometry cytek. This instrument includes 5 lasers with the competence for analysis of 18 colors. Facsfortessa is configurable and upgradeable with up to 4 lasers to detect up to 18. Bs lsrfortessa cell analyzer users guide bd biosciences. The lsr fortessa is is equipped with four laser lines 488nm, 405nm, 561nm, and.
Bd lsr ii flow cytometer indiana university bloomington. It has three spatially separated lasers 405 nm, 488 nm and 640 nm. The lsr fortessa uses bd facsflow as the sheath fluid. The software is common across bd facs instrument families, including the bd facscanto cell analyzer and bd facsaria cell sorter systems. In the flow cytometer, particles are carried to the laser intercept in a fluid stream. We work closely with other core facilities to provide comprehensive and integrative services to solve the imaging and cytometry challenges presented by cuttingedge research programs. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by bd expert training, service and supportso there is no limit to your research. Laser standby modes instruments with bd coherent connect laser control software x20s, lsr fortessa. Data shows a 10color panel run on a 3 laser bd lsrfortessa with a blueredviolet configuration. Page 2 of 6 red laser 640nm page 3 of 6 violet laser 405nm page 4 of 6 uv laser 355nm page 5 of 6 yelgr laser 561nm page 6 of 6 comments. Technical highlights lasers 355 nm, 20 mw laser power 405 nm solid state diode, 50 mw laser power 488 nm solid state, 50 mw laser power 561 nm, 640 nm, 40 mw laser power. This 5 laser instrument can distinguish many fluorescent proteins, stem cell and side populations, in addition to fluorescent antibody preparations. In addition to the bd high throughput sampler, single tube acquisition is.
Bd lsr fortessa flow cytometry facility uva school of medicine. The ability to look at multiple markers allow for better profiling of samples. Bringing boundless potential to multicolor flow cytometry, the bd lsrfortessa x20 cell analyzer gives you the power to expand and customize your science. Bd biosciences reserves the right to change its products and services at any time to. With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with bd horizon brilliant dyes, so you can focus on what matters mostyour research. Bd lsr ii users guide describes procedures necessary to operate and maintain your bd lsr ii flow cytometer. Introduction the bd high throughput sampler hts is a highspeed sampling device used on the 5 laser lsrfortessa located in rm. If the uv laser needs to be on for your experiment indo, hoechst.
The bd lsrfortessa 2 cell analyzer is a benchtop digital flow cytometer equipped with a fixedalignment flow cell that is gelcoupled to the collection optics. This video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. An advanced and flexible cell analyser with a high troughput sampler option. Page 2 of 4 red laser 640nm page 3 of 4 violet laser 405nm page 4 of 4 comments. The three laser configuration provides outstanding multiparametric data for a wide array of applications. The hts provides users the ability to acquire samples from 96well or 384well plates for efficient analysis with. The lsr fortessa is is equipped with four laser lines 488nm, 405nm, 561nm, and 640nm and is capable of detecting 18 parameters. Not for use in diagnostic or therapeutic procedures. Bd biosciences digital flow cytometry software provides. Bd lsrfortessa x20 from bd biosciences selectscience. The bd lsrfortessa 1 cell analyzer is a benchtop digital flow cytometer equipped with a fixedalignment flow cell that is gelcoupled to the collection optics. Cells are detected by digital bd facsdiva software and collected into one or as many as four tubes simultaneously from a single sample. Bd lsr ii users guide indiana university bloomington. Bd high throughput sampler users guide for the bd lsrfortessa i.
Modfit lt and quanticalc are trademarks of verity software house, inc. To log in to the facsdiva software you need to locate the icon on the desktop and. New laser options are developed on a regular basis. Bd lsrfortessa cell analyzer technical specifications. Bd facsdiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the bd lsrfortessa x20 workstation. Log in to the computer facsuser, password bdis and open bd facsdiva software. When logged in, click the bd facs diva software icon. The bd lsrfortessa x20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasersmost commonly, blue, red, violet, yellowgreen, and uvwhich provides flexibility in laser wavelengths.
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